| ZS11 Gene ID | Number of libraries expressed(TPM ≥ 1) | Mean | Min | Max | Median | Standard deviation(SD) | Coefficient of variation(CV) |
|---|
| Selector | ZS11 Gene ID | Darmor Gene ID | AtGI | At name | Genomic position | Function | Description |
|---|
| SRR | Sample name | TPM | Cultivar | Organ | Tissue | Stage | Treatment type | Treatment group | Treatment reagent | Treatment time |
|---|
ZS11: Double-low (low erucic acid and glucosinolate); ZY821: Double-high (high erucic acid and glucosinolate)
7d: 7d after flowering; 10d: 10d after flowering; 14d: 14d after flowering; 45d: 45d after flowering
T0: 24 days GET sowing; T1: 54 days GET sowing; T2: 82 days GET sowing; T3: 115 days GET sowing; T4: 147 days GET sowing
Winter ecotype: Tapidor,Quinta; Spring ecotype: Westar,No2127; Semi-winter ecotype: Shengli,Zheyou7,Gangan,ZS11
RNA-seq for leaves of all eight sequenced rapeseed accessions at five stages with one month interval including one stage before vernalization (the lowest temperature was higher than 10℃), three stages during vernalization(the lowest temperature was lower than 10℃), and one stage GET vernalization (days with low temperature lower than 10℃ were more than 100 days).
Plants were grown in unlit glasshouses, with glasshouse temperatures set at 18°C during the day and 15°C at night (approximately 16-h days). On day 22, plants were transferred to a 5°C, short-day (8 h) controlled environment room. After 6 weeks at 5°C, plants were transferred back to unlit glasshouses (approximately 16-h days) and grown until the plants flowered.
Both R-(J964) and S-lines (J902) were winte lines with similar growth periods and were grown in disease nursery plots at the experimental farm of Huazhong Agricultural University, Wuhan, China. The S. sclerotiorum isolate SS-1 was maintained and cultured on potato dextrose agar. The phenotypic characterization of the stem resistance of these two lines to S. sclerotiorum was performed in three consecutive years from 2012 to 2014 using the procedure described in our previous study. Plants were selected for inoculation and sampling using a randomized design with three biological replicates for both lines. Each replicate consisted of 30 plants for three time points (24, 48 and 96 hpi) and two treatments (inoculated and mock-inoculated controls). When the plants were at the termination of flowering, three sites on the primary stem were inoculated at three consecutive internodes (approximately 30–60 cm above the ground) with 7-mm diameter mycelial agar plugs punched from the growing margin of a 2-day-old culture of S.sclerotiorum. Mock-inoculated plants were treated with 7-mm diameter agar plugs only
BnIR is developed by the Yanglab at Huazhong Agricultural University.
Copyright © 2020-2030 All rights reserved Contact us: yqy@mail.hzau.edu.cn