In the 'Pathway' portal, eight interactive Scalable Vector Graphics (SVG) files were designed to explore candidate genes involved in the biosynthesis of terpenoid, costunolide, artemisinin, chicoric acid, and cyanidin. These secondary metabolites are either unique to the Asteraceae family or widely distributed within it. When users hover their mouse over key nodes in different metabolic pathways, basic information about the gene is displayed. Clicking on a gene reveals the corresponding candidate genes across different species. We also added interactive browsers to explore genes involved in various KEGG pathways (https://www.genome.jp/kegg/).

For the “Network” portal, users can retrieve genes co-expressed with important genes or identify their upstream/downstream regulatory target genes, thereby constructing a gene regulatory network. We have constructed the co-expression module using 74,475,396 co-expression pairs from 31 species. The co-expression network was obtained by calculating the Pearson correlation coefficient (r²) of the gene pairs side by side, and the final gene pairs r² > 0.8 were retained. Finally, we obtained 74,475,396 co-expressed gene pairs.

Furthermore, using the regulatory prediction function in PlantRegMap ^[1] (https://plantregmap.gao-lab.org/regulation_prediction.php) and based on the regulatory network of Arabidopsis, transcription factor regulatory networks for 54 species were predicted. The results yielded a total of 1,061,714 TF-gene regulatory pairs.